Amyloid β-Peptide (1-42) (human) CAS 107761-42-2

Amyloid β-Peptide (1-42) (human) CAS 107761-42-2 uses and synthesis methods

• Introduction

Soybean peptides are a mixture of polypeptides obtained by acidic or enzymatic hydrolysis or separation and refining of soybean proteins. They are mainly small molecular peptides and also contain a small amount of large molecular peptides, free amino acids, sugars and inorganic salts. The molecular weight is in Below 5000u. The protein content of soy peptides is about 85%, and its amino acid composition is the same as soy protein. It has a good balance of essential amino acids and is rich in content.

• Traits

Soybean peptides are white to slightly yellow powder. Soybean peptides have good processing properties such as no beany smell, no protein denaturation, acidity and no precipitation, no solidification when heated, easy to dissolve in water, and good fluidity. They are excellent health food materials.

• Efficacy and role

Soybean peptides have the function of quickly recovering from fatigue and enhancing muscle strength; have the function of lowering cholesterol; can treat various amino acid deficiency disorders; have the effect of promoting energy metabolism and weight loss; have the effect of lowering blood sugar; lowering blood pressure and blood lipids; other effects : Has antioxidant effect and regulates insulin function.

• Biological activity

β-Amyloid (1-42), human TFA (Amyloid β-Peptide (1-42) (human) TFA) is a 42-amino acid peptide that plays a key role in the pathogenesis of Alzheimer's disease.

• In vitro studies

β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.

2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.

3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.

4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.

Methods vary depends on the downstream applications.